Artykuły w Czytelni Medycznej o SARS-CoV-2/Covid-19

Poniżej zamieściliśmy fragment artykułu. Informacja nt. dostępu do pełnej treści artykułu tutaj
© Borgis - New Medicine 3/2009, s. 56-57
*Ewa Ogłodek1, Danuta Mo?2, Aleksander Araszkiewicz1
Effects of a 50 Hz magnetic field on the female rat´s thyroid gland in production of C cells
1Department of Clinical Psychiatry of the Nicolaus Copernicus University in Toruń
2Collegium Medicum in Bydgoszcz, Poland
2Individual medical practice
Summary
Aim: The aim of this study was to assess the effect of extremely low-frequency magnetic fields (ELF-MF) featuring magnetotherapeutic parameters used for analgesia and cell metabolism stimulation on the activity of the female rat´s thyroid gland in production of C cells synthesizing calcitonin. The ELF-MF boost secretion of calcitonin through the impact on the nerve fibres of the C cells which are located in proximity of their basement membrane.
MATERIAL AND METHOD: 24 female Wistar rats, Hannover substrain, aged 10 months, weighing 300 +/- 50 g each, were included in the experiment. Each of the rats underwent ovariectomy from a lumbar approach in the 6th month of life. The rats were divided into 2 groups: group "40”, exposed to extremely low-frequency magnetic fields of frequency 40 Hz; and control group "C” – sham exposure.
Results: In the thyroid preparations C cells and in rat´s serum calcium complex concentrations (CC-C) and calcium ion concentrations (CI-C) were evaluated.
Conclusions: The mean R-CALCY, calcium complex concentrations (CC-C) and calcium ion concentrations (CI-C) in group ´40´ were significantly different from the mean R-CALCY, CC-C and CI-C in group ´C´. ELF-MF stimulate the body to secrete calcitonin and deposit calcium in the osseous tissue. ELF-MF show a therapeutic effect on the bones, as more calcium accumulates in the bones under its influence.
INTRODUCTION
The biological effect of extremely low-frequency magnetic fields (ELF-MF) was assessed based on the model of ionic cyclotron resonance [1]. It is a theory assuming that calcium ions, which play a crucial role in cell metabolism, work as a sort of homing guidance for the impact of the magnetic field on living matter [3]. ELF-MF have an influence on ion transport, especially of calcium ones, through the calcium channels of the cytoplasmic membranes. Sustaining a calcium concentration gradient inside a cell is affected by the activity of Na+, K+/ATP and magnesium-dependent Ca++/ATP [6]. The magnesium-dependent Ca++/ATP is a catalyst for the transport of Ca++ from the cytoplasm to the extracellular space. In this study, tests were carried out on female rats which were subjected to prior experimental ovariectomy in order to achieve the effect of menopause observed in women. Menopause is connected with oestrogen-gestagen imbalance, deficit of these hormones and increased bone resorption, which leads to decrease of the BMD and the spongy bone area [9].
AIM
The aim of this study was to assess the effect of extremely low-frequency magnetic fields featuring magnetotherapeutic parameters used for analgesia and cell metabolism stimulation on the activity of the female rat´s thyroid gland in production of C cells synthesizing calcitonin.
MATERIAL AND METHOD
24 female Wistar rats, Hannover substrain, aged 10 months, weighing 300 +/- 50 g each, were included in the experiment. Each of the rats underwent ovariectomy from a lumbar approach in the 6th month of life. The rats were divided into 2 groups: group "40”, exposed to extremely low-frequency magnetic fields of frequency 40 Hz; and control group "C” – sham exposure. Microscopic slides were made from the rats´ thyroid glands. After rinsing the preparations in PBS buffer solution, they were marked with an antibody against calcitonin leading to induction of an immunoenzymatic reaction, which resulted in brown staining of the C cells. A quantitative analysis of the C cells in the prepared slides was performed consisting in evaluation of 50 view fields of each of the slides. In the rat serum were evaluated calcium complex concentrations (CC-C) and calcium ion concentrations (CI-C).
The following methods were used for the purpose of the statistical analysis: ANOVA for a single variable was performed using the following tests: Cochran-Cox test and investigation of significance of differences between the mean values (post-hoc analysis) by means of Tukey´s test.
RESULTS

Powyżej zamieściliśmy fragment artykułu, do którego możesz uzyskać pełny dostęp.

Płatny dostęp tylko do jednego, POWYŻSZEGO artykułu w Czytelni Medycznej
(uzyskany kod musi być wprowadzony na stronie artykułu, do którego został wykupiony)

Aby uzyskać płatny dostęp do pełnej treści powyższego artykułu wraz z piśmiennictwem , należy wprowadzić kod:

Kod (cena 19 zł za 7 dni dostępu) mogą Państwo uzyskać, przechodząc na tę stronę.
Wprowadzając kod, akceptują Państwo treść Regulaminu oraz potwierdzają zapoznanie się z nim.

 

 

Płatny dostęp do wszystkich zasobów Czytelni Medycznej

Aby uzyskać płatny dostęp do pełnej treści powyższego artykułu wraz z piśmiennictwem oraz WSZYSTKICH około 7000 artykułów Czytelni, należy wprowadzić kod:

Kod (cena 49 zł za 30 dni dostępu) mogą Państwo uzyskać, przechodząc na tę stronę.
Wprowadzając kod, akceptują Państwo treść Regulaminu oraz potwierdzają zapoznanie się z nim.

Adres do korespondencji:
*Ewa Ogłodek
Department of Psychiatry, Nicolaus Copernicus University, Toruń,
Medical University of Bydgoszcz, Kurpińskiego Str. 19, Bydgoszcz, 85-096
tel.: 0-669-300-460
e-mail: maxeve@interia.pl

New Medicine 3/2009
Strona internetowa czasopisma New Medicine