© Borgis - Nowa Medycyna 2/2009, s. 149-150
Respiratory Viruses – their detection and significance in respiratory infections
Department of Clinical Microbiology, Hvidovre University Hospital
Acute respiratory disease causes the majority of morbidities in the community in temperate countries. The contribution of respiratory viruses of to the disease burden in the community as well as in the hospital setting is becoming well recognized (Tsolia et al, 2004; Jennings et al., 2007; Johnstone et al., 2008) as methods for their detection has become increasingly refined. Virus culture and especially the application of molecular techniques to clinical samples in the research setting has led to and the description of novel respiratory virus as possible agents causing human disease. Examples include human coronavirus NL63 (Fouchier et al., 2004) and HKU1 (Woo et al., 2005), human Bocavirus (Allander et al., 2005), polyomavirus Wu (Allander et al., 2007) and Ki (Gaynor et al., 2007). The application of molecular techniques to clinical samples has also led to the description of new syndromes caused by well known agents. For example Pneumocystis jirovecii, now generally accepted as a fungus, is associated with a "viral-like” self-resolving syndrome in immunocompetent infants (Larsen et al., 2007). To further complicate the matter co-infections are frequent and evidence suggest that viral and bacterial coinfection may cause an synergistic increase in virulence (Bakeletz, 1995; Jennings et al., 2008). The time interval between the description of a novel agent and the possible application of a diagnostic test in a clinical setting has considerably shortened with the advent of molecular tests. This advantageous development has in turn yielded less time for clinical evidence to accumulate and this in turn makes clinical interpretation of test results more difficult. In this review, I will discuss recent advances in molecular detection of respiratory viruses, how testing is done typically done in Denmark with special reference to my own laboratory and finally discuss the problems of clinical interpretation of new diagnostic tests of new agents using human bocavirus as an example.
Virus isolation has been a golden standard due to its inherent high specificity, however, in Denmark it has never been established as a routine diagnostic test due to high turn-around time, high cost and labour intensiveness. Serological tests have been valuable in seroepidemiological surveys, but has had limited impact in routine clinical testing as paired sera are seldom obtained. Direct fluorescent antibody (DFA) tests have been largely abandoned and replaced by molecular methods. ELISA and immunochromatographic antigen demonstration of virus-Ag is still important as rapid tests and point of care-tests in Denmark.
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