Ludzkie koronawirusy - autor: Krzysztof Pyrć z Zakładu Mikrobiologii, Wydział Biochemii, Biofizyki i Biotechnologii, Uniwersytet Jagielloński, Kraków

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© Borgis - New Medicine 1/2010, s. 2-6
*Petr Kutáč
Reliability of body composition measurement by the BIA Method (bioelectric impedance)
Centre of Human Motion Diagnostics – Department of Physical Education, Pedagogical Faculty, University of Ostrava, Czech Republic
Head of Department: Doc. PhDr. Vojtěch Gajda, CSc.
Summary
Introduction. The basic condition for credibility of the measured values for all the used methods is their standardization. Aside from complying with standard measuring conditions it includes especially knowledge of the method´s validity and reliability.
Aim. The aim of this study is to estimate the specific reliability of body composition measurement by the BIA Method (using TANITA 418 MA device), by means of its parallel form generated by multiple repetition of the given measurement.
Material and methods. The experimental sample group comprised of 91 students of Physical Education (53 men and 38 women) whose average age was 20.5 and 20.1 years, respectively. A tetrapolar bioimpedance weighing machine TANITA 418 MA was used for measurement of body fat ratio by the BIA method. For estimation of the specific reliability we used a calculation of correlation coefficient between two parallel forms of measurement. All the statistic tests were realized on a significance level α/symbol> = 0.05.
Results. The values of Pearson correlation coefficient (rXX') exceeded 0.970 in all tested parameters. The value of body weight was calculated as 1.000. The values of standard test error (SE) for monitored parameters oscillated between 0.06-0.12.
Conclusion. With regard to the high values of rXX' and low values of SE we are able to assess the impact ratio of the external intervention affecting the changes in body composition even in relatively small changes of the monitored parameters. The basic condition for this is a strict complying with the standard measurement conditions.
Introduction
Apart from the body height, we can include among the basic anthropometric characteristics also the body weight. For this reason, the body weight is, in most studies, one of the main characteristics of the monitored sample. However, the body weight is a relatively complex parameter which is composed of a series of fractions (components) (1, 2, 3). Therefore, when we want to assess whether an individual has an appropriate body weight, using only some weight-height index without assessment of the individual body weight fractions can be, in many cases, misleading, and it can serve only as a draft assessment, rather for average population. In the sport sphere we can find cases when the BMI levels indicate overweight in the monitored sportsman, sometimes on the verge of obesity. These individuals have a low fat ratio though, and their increased weight is caused by an intensive development of the muscle-skeletal apparatus (4, 5, 6, 7). Therefore it is necessary to examine the proportion of the individual fractions (body composition) (8). Body composition is presently a largely monitored parameter, because it is considered to be an indicator of the individual´s health condition, his nutrition level and physical fitness (9, 10). In sports, it can – to a certain degree – determine the readiness level of the sportsman´s organism for the training and race body burden. And therefore we can consider it to be one of the key aspects of the sport performance. By means of monitoring the body composition changes we can assess the influence of the physical exercise (training burden) on the sportsman´s organism. For this reason the body composition determination is an integral part of the sportsmen diagnostics during functionality examinations (11, 12, 13, 14). The most often monitored fraction is body fat. In sport practice, some trainers and specialists consider the 1% change of body fat ratio significant (8, 15).
When determining body composition it is always necessary to measure a series of parameters by means of various methods which can be divided into laboratory methods and field methods. Selected laboratory methods serve at the same time as reference methods. In conditions of a field practice they are demanding in terms of technical equipment, staff skills requirements, organization and last, but not least, costs (16, 17, 18). Therefore, in field practice we use field methods which allow field examination of the large samples – they are not demanding in terms of organization, staff training and they are also affordably priced. Anthropometry can be also included into these methods and, especially, bioelectric impedance method (BIA), which is now very popular. This method works on the basis of different flow of a low-intensity electric current in various biological structures. It is based on the principle of different electric characteristics of tissues, fat and mainly body water. The current flows through water and through electrolyte components in fat free mass and the resulting resistance is therefore proportional to its volume (17). Individual fractions of the body weight are calculated from the values of impedance on the basis of regression equations (19).
The basic condition for credibility of the measured values at all used methods is their standardization. Apart from complying with the standard measurement conditions (which are described both in treatises and in measuring device manuals), it includes especially knowledge of a method´s validity and reliability (20).
The presented paper deals with the BIA method realized by means of Tanita BC – 418 device. The issue of validity of the BIA method realized by means of this device has been already solved (21), therefore this paper focuses mainly on the reliability of the method.
Aim
The main aim of this study is to estimate the specific reliability of body composition measurement by the BIA Method (using TANITA 418 MA device), by means of its parallel form generated by multiple repetition of the given measurement. And, consequently, to assess the factual acceptability of errors for this measurement in monitored parameters of body composition.
Material and methods
The experimental sample group comprised of 91 students specializing in Physical Education and Sports at Pedagogical Faculty, University of Ostrava. These individuals, with regard to their specialization, can be considered as representatives of a healthy population. The total of tested students was 91 (53 men and 38 women). Average age was 20.5 years in men and 20.1 years in women. A tetrapolar bioimpedance weighing machine TANITA 418 MA was used for measurement of body fat ration by the BIA method. The measurement complied with all recommended rules. It was performed in a STANDARD regime because there was not detected 10 or more hours of weekly motion activities in any tested person (which is a condition for selecting an ATHLETIC regime).
Body composition is represented by values of body weight, body fat and total body water. Fat-free mass was not detected because its value was calculated as a difference of body weight and body fat.
The estimation of the specific reliability was based on the classical model of test theory (22), where a calculation of correlation coefficient between two parallel forms of measurement was used for the reliability estimation. We used a method of multiple repetition of the given measurement for creating of the parallel form. And we used the same theory for calculation of the test error.
When evaluating results of body fat measurement and total body water measurement the resulting values for three groups were assessed: 1st group consisted only of men, 2nd group consisted only of women and 3rd group consisted of all the tested persons regardless of their sex. It allowed to assess a diagnostic error of the method between groups with a different ratio of a fat fraction. First and second measurements were performed consecutively (within the range of 10 minutes) so that the result could not be affected by the external factors (e.g. water intake etc.).
The statistical computing was performed by means of PASW SPSS 18.0 statistical software. All the statistic tests were realized on a significance level α = 0.05.
The study protocol was approved by the Ethics and Research Committee of the University of Ostrava. All participants signed an informed consent form.
Results
a) Body fat measurement

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Piśmiennictwo
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otrzymano: 2009-11-16
zaakceptowano do druku: 2010-01-04

Adres do korespondencji:
*Petr Kutáč
KTV PdF OU, Varenská 40a, Ostrava 1
702 00, Czech Republic
phone: +420 596613837
e-mail: petr.kutac@osu.cz

New Medicine 1/2010
Strona internetowa czasopisma New Medicine