Ludzkie koronawirusy - autor: Krzysztof Pyrć z Zakładu Mikrobiologii, Wydział Biochemii, Biofizyki i Biotechnologii, Uniwersytet Jagielloński, Kraków

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© Borgis - Postępy Nauk Medycznych 9/2012, s. 699-704
*Krzysztof Jamroziak1, Olga Grzybowska-Izydorczyk2, Anna Szmigielska-Kapłon1, Dorota Jesionek-Kupnicka3, Ewa Wawrzyniak1, Barbara Pieńkowska-Grela4, Tadeusz Robak1
Nowotwory z blastycznych plazmacytoidalnych komórek dendrytycznych – opis dwóch przypadków
Blastic plasmacytoid dendritic cell neoplasms – a report of two cases
1Department of Hematology, Medical University of Łódź
Head of Department: prof. Tadeusz Robak, MD, PhD
2Department of Experimental Hematology, Medical University of Łódź
Head of Department: prof. Piotr Smolewski, MD, PhD
3Department of Pathology, Medical University of Łódź
Head of Department: prof. Radzisław Kordek, MD, PhD
4Department of Cytogenetic, The Maria Skłodowska-Curie Memorial Cancer Center and Institute, Warsaw
Head of Department: Barbara Pieńkowska-Grela, MD, PhD
Streszczenie
Artykuł zawiera opis dwóch przypadków chorych z rozpoznaniem bardzo rzadko występujących nowotworów z blastycznych plazmacytoidalnych komórek dendrytycznych (blastic plasmacytoid dendritic cell neoplasms – BPDCN). BPDCN charakteryzują się koekspresją antygenów CD4 i CD56 przy braku ekspresji innych mieloidalnych lub limfoidalnych markerów liniowych, pierwotnym zajęciem skóry i wtórnym szpiku kostnego oraz agresywnym przebiegiem klinicznym. Rokowanie jest złe, szczególnie w przypadku leczenia konwencjonalną chemioterapią. Zgodnie z opublikowanymi danymi jedyną opcją terapeutyczną, która wydaje się poprawiać rokowanie w BPDCN jest intensywna chemioterapia skojarzona z allogenicznym przeszczepieniem macierzystych komórek hematopoetycznych.
Summary
In this paper we report two cases of very rare blastic plasmacytoid dendritic cell neoplasms (BPDCN). BPDCN are characterized by co-expression of CD4 and CD56 antigens in the absence of any specific myeloid or lymphoid lineage or markers, primary skin infiltrations followed by bone marrow involvement, and aggressive clinical course. The prognosis is poor, especially when treatment is based on conventional chemotherapy. According to published evidence the only treatment with some potential to improve BPDCN outcome is intensive chemotherapy consolidated by allogeneic hematopoietic stem cell transplantation.
Introduction
A category of rare and aggressive haematologic tumors co-expressing CD4 and CD56 antigens in the absence of any specific myeloid, B-, T-lymphoid or natural killer (NK) lineage markers is classified as blastic plasmacytoid dendritic cell neoplasms (BPDCN) according to the most recent 2008 WHO classification of tumours of haematopoietic and lymphoid tissues (1). This is in line with the recent knowledge that BPDCN are derived from the precursors of plasmacytoid dentritic cells (2-4). However, in the past a number of different terms were used for BPDCN due to their uncommon immunophenotypic, histopathological and clinical features, including blastic NK leukemia/lymphoma, histiocytic lymphoma or histiocytic associated hematologic malignancy, cutaneous a granular CD4+ CD56+ hematodermic neoplasm and NK-cell lymphoma or myelo-monocytic precursor – related lymphoma (5-12).
BPDCN are diagnosed about three times more often among men than women, mainly in the elderly (median age at diagnosis at 7th decade of live) (5, 10). Till present the etiology of BPDCN has not been elucidated. Epstein Barr virus and HIV had been previously supposed to play a role in BPDCN pathogenesis, but later reports did not confirm their correlation to the disease development (5-12). Clinically, the typical initial presentation of BPDCN is presence of asymptomatic cutaneous lesions that are subsequently followed by bone marrow involvement and leukemic dissemination (5, 10). The disease is characterized by highly aggressive clinical course. Although BPDCN respond to different types of chemotherapy, the responses are usually of short duration, and disease is most often fatal within a few years from diagnosis (5, 10).
Despite rare incidence of BPDCN, numerous variations from typical clinicopathological picture of BPDCN have been reported, such as different extracutaneous manifestation, lack of skin involvement, immunophenotypic variants or other, making a diagnosis even more complicated (13-17). In this paper we report two cases of BPDCN diagnosed in our center with objective to increase clinical awareness for these rare and aggressive malignancies that seem to be underdiagnosed in Poland.
Case 1
63-year old man was referred to the Department of Chemotherapy of Medical University of Łódź in April 2008 for evaluation of potentially malignant skin lesions. The history of B symptoms was negative. On examination, the skin lesions were found to be predominantly purpuric nodules, in association with bruise-like areas, and were distributed over the left forearm. The majority of the nodules ranged from 1 to 2 cm in diameter. The nodules were not painful on palpation and the temperature of the adjacent skin was normal. On the physical examination lymphadenopathy, hepatomegaly or splenomagaly were excluded. The computed tomography scan of the chest and abdomen revealed no enlarged lymph nodes in the mediastinum or retroperitoneum. The complete blood count (CBC) was normal.
On the basis of histopathological and immunohistochemical examination of the full-layer skin biopsy the diagnosis of BPDCN was established (fig. 1A-D). The skin was shown to be involved by dense, diffuse infiltration of blastic cells with irregular nuclei and one to a few nucleoli (fig. 1D).
Fig. 1. The histopathological features of blastic plasmacytoid dendritic cell neoplasm infiltrating the skin in the case 1 (A, C) and case 2 (B, D). The infiltrates are dense and diffuse, save the epidermis (A) but deeply invade the adipose tissue (B and C). The blastic cells are medium-sized with variable shape of nuclei and various number of nucleoli; the mitotic figures are seen (D).
The epidermis was intact, while the infiltration was extended to subcutaneous fat (fig. 1 A, B, C). The variable number of mitoses was seen (fig. 1D). Immunohistochemical profile was determined as positive for LCA, CD4, CD43 and CD56, and negative for such lineage markers as CD3, CD8, CD20, CD30, myeloperoxidase (MPO) and terminal deoxynucleotidyltransferase (TdT) andgranzyme B. For differential diagnosis with other small blue round cell tumors staining with other markers were also performed, namely CD99 (-), CKMNF (-), CK20 (-), HMB45 (-), NSE-, TTF1 (-), CKAE1/AE3 (-), Melan A (-), Vimentin (+), and synaptophysin (-). Proliferative index of Ki -67 (MIB-1) reached 80%. Additionally, bone marrow aspiration was performed to exclude bone marrow involvement by BPDCN and shown normal marrow cytology. The treatment was based on standard chemotherapy protocol CHOP (cyclophosphamide, doxorubicine, vincristine and prednisone) protocol. The patient received seven CHOP cycles at 4-week intervals and achieved complete resolution of skin infiltrations.
In September 2008 the patient was referred to our center (Department of Hematology of Medical University of Łódź) due to suspicion of tumor relapse. Clinical examination disclosed reappearance of bluish-red nodular changes (diameter of 1 cm) on the left forearm and painful bulky lymph nodes in the right inguinal region (maximal dimensions of 10 and 6 cm). The history of B symptoms was negative. The liver and spleen were not palpable. CBC revealed mild anemia with hemoglobin concentration of 11 g/dl and normal white blood cells (WBC 4.11 x 10^3/ul) and platelets counts (PLT 234 x 10^3/ul). Skin lesion biopsy was performed and the histological and immunohistochemical examinations confirmed relapse of BPDCN. Immunophenotypig of tumor cells sampled through inguinal lymph node biopsy showed positive CD4, CD56, CD123 and BDCA-2 antigens with negative CD34 antigen and myeloid or lymphoid lineage specific antigens reflecting infiltration by BPDCN. Moreover, cells taken through inguinal lymph node biopsy were analyzed for cytogenetic abnormalities using 24-hours unstimulated culture and G-banding technique and karyotype was described according to the International System for Human Cytogenetic Nomenclature (18). This analysis revealed multiple cytogenetic aberrations with karyotype description as follows: 48-50, XY, +der(8)t(1;8)(q21;p11), del(10)(q2?2),+1~3mar[cp10]/46,XY[3]. The aspirated bone marrow sample was normocellular with 33% erythroid precursors, 53% leucocytic precursors, 9.5% lymphocytes, 1.5% plasmocytes, 0.5% megakaryocytes and 1.5% monocytes. No atypical cells were found in the bone marrow during cytological inspection. The examination of cerebrospinal fluid did not reveal any abnormalities.
Since rapid disease recurrence was seen despite previously administered lymphoma-type chemotherapy, the treatment strategy was changed to intensive chemotherapy used in acute myelogenous leukemia (AML). An induction regimen DAC-7 (daunorubicin, cytarabine, and cladribine) was administered without post-chemotherapy complications (19). Additionally, methotrexate, cytarabineand dexamethasone were delivered intrathecally as prophylaxis of central nervous system (CNS) involvement. However, only temporary partial response was achieved with partial resolution of skin infiltrations and reduction of the size of the inguinal lymph nodes to 3 cm.

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Piśmiennictwo
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otrzymano: 2012-06-20
zaakceptowano do druku: 2012-07-18

Adres do korespondencji:
*Krzysztof Jamroziak
Department of Hematology Medical University of Łódź
ul. Ciołkowskiego 2, 93-510 Łódź
tel.: +48 (42) 689-51-91, fax: +48 (42) 689-51-91
e-mail: krzysztof.jamroziak@wp.pl

Postępy Nauk Medycznych 9/2012
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