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© Borgis - New Medicine 3/2009, s. 58-60
*Ewa Ogłodek1, Danuta Mo?1, 2
The Effect of Extremely Low Frequency Magnetic Fields on Osteogenesis
1Department of Clinical Psychiatry of the Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Poland
Head of Clinic: prof. dr hab med. Aleksander Araszkiewicz
2Individual medical practice
Summary
Extremely low frequency magnetic fields (ELF-MF) which are applied in the treatment of humans are distinguished from various sorts of magnetic fields. They are magnetic fields of frequency not higher than 60 Hz, induction value ranging from 1 pT to 15 mT, with volume of 130 V/m, and both rectangular and triangular waveform of the magnetic field. The essential problem raised is the effect of the magnetic field on particular metabolic processes of living organisms. The long-term exposure of an organism to extremely low frequency magnetic fields intensifies the process of membrane transmission, resulting in morphological changes in the bone. The use of magnetic fields is gaining acceptance for the treatment of ununited fractures. As a reaction to the stress stimulus in the form of extremely low frequency magnetic fields activity, an increase of biosynthesis of cellular proteins and of transcription in a cell are observed and also changes in expression and differentiation of genes occur.
Extremely low frequency magnetic fields stimulate bone formation by promoting osteoblastic differentiation and activation. In the matrix development and mineralization stages, the calcium content in the matrix and two markers of osteoblastic phenotype (alkaline phosphatase and osteocalcin) also showed a significant increase. The objective of this research was to evaluate the influence of extremely low frequency magnetic fields on osteogenesis.



Extremely low frequency magnetic fields have now been in use in orthopaedics and traumatology for over twenty years [1, 2]. Clinical trials have shown that magnetic stimulation enhances calcification of bones [3]. The technique is employed to promote osteogenesis and hence to favour bone repair processes. It has recently been shown that extremely low frequency magnetic field (ELF-MF) may be the treatment of choice in avascular necrosis of the femoral head. The effect of ELF-MF on the proliferative activity of osteoblasts in vitro and on the speed of the osteogenic repair process in vivo is more evident and ample when cultures of cells are taken from elderly donors or when the examined subject is in advanced age [4, 5].
The objective of this study was to evaluate the influence of extremely low frequency magnetic fields on the growth of osteoblast cells.
Mechanism of the effect of extremely low frequency magnetic field on bone cells
The proliferation and differentiation, which are responsible for growth, remodelling, and repair of bones are modulated by several extracellular factors, such as cytokines and hormones [6]. Bone formation is also affected by pulsed magnetic fields. Extremely low frequency magnetic fields are extensively applied in the clinical treatment of non-union bone fractures, bone grafts, fresh fractures and osteoporosis. As for the effects of pulsed electromagnetic fields on bone, much evidence suggests that they enhance the activities of osteoblasts, proliferation and differentiation, extracellular matrices, alkaline phosphatase and net flux and the uptake of calcium [7, 8].
While several different mechanisms may contribute to the overall healing effectiveness of ELF-MF (increased proliferation and differentiation of pre-osteogenic cells, increased blood flow, increased ion concentration in bone), altered hormone receptor activity seems to play a pivotal role [9, 10]. Osteoblasts exposed for as little as 10 min to pulsed ELF-MF with peak fields in the range of 1 mT show persistent desensitization of the PTH receptor resulting in a rate of collagen synthesis elevated by the osteoblasts, and a rate of bone resorption decreased by osteoclasts [11, 12]. Exposure to the extremely low frequency magnetic field caused increased calcium, leading to phosphorylation of membrane proteins, PKC-induced desensitization of the PTH receptor, and other signal transduction to the cellular membrane. The ELF-MF caused changes in the charge and/or affinity properties of membrane proteins [13, 14].

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Piśmiennictwo
1. Coquillat PB: Use of magnetic fields in medicine. Rev Clin Esp. 1996; 196(2): 63-5. 2. Rubin CT et al.: Prevention of osteoporosis by pulsed electromagnetic fields. J Bone Joint Surg Am. 1989; 71(3): 411-7. 3. Satter Syed A et al.: Pulsed electromagnetic fields for the treatment of bone fractures. Bangladesh Med Res Counc Bull. 1999; 25(1): 6-10. 4. Canč V et al.: Pulsed magnetic fields improve osteoblast activity during the repair of an experimental osseous defect. J Orthop Res. 1993; 11(5): 664-70. 5. Icaro Cornaglia A et al.: Stimulation of osteoblast growth by an electromagnetic field in a model of bone-like construct. Eur J Histochem. 2006; 50(3): 199-204. 6. Wei Y et al.: Effects of extremely low-frequency-pulsed electromagnetic field on different-derived osteoblast-like cells. Electromagn Biol Med. 2008; 27(3): 298-311. 7. Ciombor DM et al.: Low frequency EMF regulates chondrocyte differentiation and expression of matrix proteins. J Orthop Res. 2002; 20(1): 40-50. 8. Haupt HA. Electrical stimulation of osteogenesis. South Med J. 1984; 77(1): 56-64. 9. Kesemenli CC et al.: The effects of electromagnetic field on distraction osteogenesis. Yonsei Med J. 2003; 44(3): 385-91. 10. Mc Leod KJ et al.: The effect of low-frequency electrical fields on osteogenesis. J Bone Joint Surg Am. 1992; 74(6): 920-9. 11. Werhahn C.: Biophysical foundations in the application of electromagnetic fields in the modification of osteogenesis. Z Orthop Ihre Grenzgeb. 1991; 129(1): 118-25. 12. De Mattei M et al.: Effects of pulsed electromagnetic fields on human articular chondrocyte proliferation. Connect Tissue Res. 2001; 42(4): 269–79. 13. De Mattei M et al.: Effects of electromagnetic fields on proteoglycan metabolism of bovine articular cartilage explants. Connect Tissue Res. 2003; 44: 154-9. 14. Pezzetti F et al.: Effects of pulsed electromagnetic fields on human chondrocytes: an in vitro study. Calcif Tissue Int. 1999; 65: 396-401. 15. Varani K et al.: Characterization of adenosine receptors in bovine chondrocytes and fibroblast-like synoviocytes exposed to low frequency low energy pulsed electromagnetic fields. Osteoarthr Cartil. 2008; 16(3): 292-304. 16. Varani K et al.: Effect of low frequency electromagnetic fields on A2A adenosine receptors in human neutrophils. Br J Pharmacol. 2002; 136: 57-66. 17. Ciombor DM et al.: Modification of osteoarthritis by pulsed electromagnetic field-a morphological study. Osteoarthr Cartil. 2003; 11(6): 455-62. 18. Fini M et al.: Pulsed electromagnetic fields reduce knee osteoarthritic lesion progression in the aged Dunkin Hartley guinea pig. J Orthop Res. 2005; 23(4): 899-908. 19. Cohen SB et al.: Reducing joint destruction due to septic arthrosis using an adenosine2A receptor agonist. J Orthop Res. 2004, 22: 427-435. 20. Ulrich-Vinther M et al.: Articular cartilage biology. J Am Acad Orthop Surg. 2003; 11(6): 421-30. 21. Varani K et al.: Effect of low frequency electromagnetic fields on A2A adenosine receptors in human neutrophils. Br J Pharmacol. 2002; 136: 57-66. 22. Farrell E et al.: Cell labelling with superparamagnetic iron oxide has no effect on chondrocyte behaviour. Osteoarthritis Cartilage. 2009; 17(7): 958-64. 23. Schulz RM et al.: Development and validation of a novel bioreactor system for load- and perfusion-controlled tissue engineering of chondrocyte-constructs. Biotechnol Bioeng. 2008; 101(4): 714-28. 24. Schmidt-Rohlfing B et al.: Effects of pulsed and sinusoid electromagnetic fields on human chondrocytes cultivated in a collagen matrix. Rheumatol Int. 2008; 28(10): 971-7. 25. Benazzo F et al.: Effects of biophysical stimulation in patients undergoing arthroscopic reconstruction of anterior cruciate ligament: prospective, randomized and double blind study. Knee Surg Sports Traumatol Arthrosc. 2008; 16(6): 595-601. 26. Bachl N et al.: Electromagnetic interventions in musculoskeletal disorders. Clin Sports Med. 2008; 27(1): 87-105. 27. Massari L et al.: Effects of electrical physical stimuli on articular cartilage. J Bone Joint Surg Am. 2007; 89(Suppl 3): 152-61. 28. Jahns M et al.: A programmable ramp waveform generator for PEMF exposure studies on chondrocytes. Eng Med Biol Soc. 2006; 1: 3230-3. 29. Nicolin V et al.: In vitro exposure of human chondrocytes to pulsed electromagnetic fields. Eur J Histochem. 2007; 51(3): 203-12. 30. Hsieh CH et al.: Deleterious effects of MRI on chondrocytes. Osteoarthritis Cartilage. 2008; 16(3): 343-51. 31. Ciombor DM et al.: Low frequency EMF regulates chondrocyte differentiation and expression of matrix proteins. J Orthop Res. 2002; 20(1): 40-50. 32. Stolfa S et al.: Effects of static magnetic field and pulsed electromagnetic field on viability of human chondrocytes in vitro. Physiol Res. 2007; 56(Suppl 1): 45-9. 33. Sunk IG et al.: Impairment of chondrocyte biosynthetic activity by exposure to 3-tesla high-field magnetic resonance imaging is temporary. Arthritis Res Ther. 2006; 8(4): 106. 34. Neu CP et al.: Heterogeneous three-dimensional strain fields during unconfined cyclic compression in bovine articular cartilage explants. J Orthop Res. 2005; 23(6): 1390-8. 35. Elliott JP et al.: Time-varying magnetic fields: effects of orientation on chondrocyte proliferation. J Orthop Res. 1988; 6(2): 259-64.
Adres do korespondencji:
*Ewa Ogłodek
Department of Psychiatry, Nicolaus Copernicus University, Toruń,
Medical University of Bydgoszcz, Kurpińskiego Str. 19, Bydgoszcz, 85-096
tel.: 0-669-300-460
e-mail: maxeve@interia.pl

New Medicine 3/2009
Strona internetowa czasopisma New Medicine